Biology 107 Lab

DNA

Name _________________

Section_________________

During the DNA extraction, the strawberry was treated with a extraction buffer the material was filtered through a coffee filter.

1. How did the extraction buffer work? (Why were the chromosomes liberated from the cells of the strawberry?)

2. What was the purpose of the coffee filter?

3. Is the DNA found in all living cells (bacteria, plants, animals, etc.)?

4. Write the complementary DNA strand sequence to the sequence below:

            T         A         C         C         T         A         G         G          A        C         C         A

            ___      ___      ___      ___      ___      ___      ___      ___      ___      ___      ___      ___ 

5.         When messenger RNA reads the DNA sequence above, what would be the proper order of nitrogen bases?

            T         A         C         C         T         A         G         G          A        C         C         A

            ___      ___      ___      ___      ___      ___      ___      ___      ___      ___      ___      ___ 

6. The above process of reading the DNA sequence and converting it to RNA sequence is called:

            _________________________

7. The conversion of the RNA code to the sequence of amino acids is called:_________________ 

Figure 1

8. What is the molecule in Figure 1: ______________________

9. What base is unique to this (Figure 1) molecule? ________________

Figure 2

10. Figure 2 represents what molecule? _________________________

11. Name two differences between Figure 1 and Figure 2:

            a. _________________________________________________________

            b. _________________________________________________________

12. On the next page is a copy of the genetic code for the amino acids. Using the answers for number 5 on page 1, what are the first two amino acids using the RNA code.

            a. __________________________

            b. ___________________________

13. What was the purpose of the restriction enzyme card in the exercise dealing with DNA finger printing?

            ____________________________________________________________________

14. What was the purpose of using a radioactive probe during the gel runs?

_________________________________________________________________________

15.Which fragments are more likely to end up at the top of the gel plate (small ones or large ones)?

            ______________________________

16. Beside solving crimes, what would be a practical purpose for DNA finger printing techniques?

            _______________________________________________________________________