1. Were the absorbency readings directly or indirectly proportional to the concentration in the reaction mixtures:

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2. In the experiment on varying the amount of enzyme concentration, would you expect the plot to continue upward, level off (stops) or go downward if excess enzyme were added?

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3. What would have happened, if you had omitted substrate from the reaction mixture?
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4. What is the relationship between Ph and the rate of substrate breakdown?

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5. What was the pH optimum for alkaline phosphatase? __________________

6. Why was NaOH added to the reaction mixture? ________________________

7. In the ‘effect of temperature experiment’, why was the enzyme boiled?

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8. In general, what is the relationship between temperature and the rate of substrate breakdown?
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9. If a reaction involved the following steps: A > B > C > D, how many enzymes would have been involved?
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10. What is the topic of Today's Lab? _____________________________